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N6-Methyladenosine Modification Regulates Prunus Necrotic Ringspot Virus Infection in Cucumis sativus
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N6-Methyladenosine Modification Regulates Prunus Necrotic Ringspot Virus Infection in Cucumis sativus
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  • Zhenfei Dong
    Zhenfei Dong
    Department of Fruit Science, College of Horticulture, China Agricultural University, Beijing 100193, China
    State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
    More by Zhenfei Dong
  • Na Liu
    Na Liu
    State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
    More by Na Liu
  • Zhixiang Zhang
    Zhixiang Zhang
    State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
    More by Zhixiang Zhang
  • Hongqing Wang
    Hongqing Wang
    Department of Fruit Science, College of Horticulture, China Agricultural University, Beijing 100193, China
    More by Hongqing Wang
  • Shifang Li*
    Shifang Li
    State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
    Center for Biosafety, Chinese Academy of Inspection and Quarantine, Sanya 572024, Hainan, China
    *Email: [email protected]
    More by Shifang Li
  • Binhui Zhan*
    Binhui Zhan
    State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
    *Email: [email protected]
    More by Binhui Zhan
    Orcidhttps://orcid.org/0000-0002-2509-2509
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Journal of Agricultural and Food Chemistry

Cite this: J. Agric. Food Chem. 2025, 73, 19, 11586–11597
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https://doi.org/10.1021/acs.jafc.5c00050
Published May 4, 2025
Copyright © 2025 American Chemical Society
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Abstract

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N6-methyladenosine (m6A) is a common epigenetic modification found in eukaryotic RNA. Recent studies have increasingly highlighted the importance of m6A modification in plant defense against viruses. In this investigation, we found that prunus necrotic ringspot virus (PNRSV) infection affected the m6A modification process. Plant transcriptomes and epitranscriptomes were sequenced to coanalyze the dynamic changes of m6A modifications after PNRSV infection. Further studies revealed that the silencing of evolutionarily conserved C-terminal region (ECTs), encoding m6A readers, led to increased PNRSV accumulation, indicating that ECTs confer resistance to PNRSV. Additionally, we demonstrated that UPF3 and SMG7, which are involved in non-sense-mediated mRNA decay pathways, as well as phenylalanine ammonia-lyase (PAL), a well-known key enzyme in plant defense and an identified m6A-modified gene following PNRSV infection, play crucial roles in regulating PNRSV infection. These findings provide new insights into understanding PNRSV infection and further elucidate the role of m6A in modulating viral infection in plants.

Copyright © 2025 American Chemical Society

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Supporting Information

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The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.jafc.5c00050.

  • Symptom of PNRSV infection in Cucumis sativus (Figure S1); distribution of alignment regions in the reference genome by MeRIP-seq (Figure S2); distribution of reads on the gene by MeRIP-seq (Figure S3); validate the accuracy of MeRIP-seq data (Figure S4); analysis of differentially expressed genes (DEGs) between mock- and PNRSV-inoculated samples (Figure S5); GO and KEGG analysis of differentially expressed genes (DEGs) between mock- and PNRSV-inoculated samples (Figure S6) (PDF)

  • Specific primers used for PNRSV detection and VIGS assays-primers (Table S1); sequenced and genome-mapped reads (Table S2); Mock-IP VS Mock-input peak (Table S3); PNRSV-IP VS PNRSV-input peak (Table S4); PNRSV VS Mock diffPeak result (Table S5); PNRSV-input VS Mock-input-Gene differential expression (Table S6); and PNRSV VS Mock all diffPeak Gene differential expression (Table S7) (XLSX)

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Cited By

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This article is cited by 1 publications.

  1. Rakesh Srivastava, Niraj Lodhi. Role of m6A mRNA Methylation in Plant Defense. Epigenomes 2025, 9 (4) , 42. https://doi.org/10.3390/epigenomes9040042
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Journal of Agricultural and Food Chemistry

Cite this: J. Agric. Food Chem. 2025, 73, 19, 11586–11597
Click to copy citationCitation copied!
https://doi.org/10.1021/acs.jafc.5c00050
Published May 4, 2025
Copyright © 2025 American Chemical Society
Request reuse permissions

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